ISSN 2398-2969      

Staining technique: Ziehl-Neelsen

Clapis

Synonym(s): Acid fast


Introduction

  • Used to detect acid fast organisms, such asMycobacteriumspp.
  • Acid fast bacilli stain bright red due to the presence of mycolic acid in their cell wall, the background and other bacteria stain green or blue depending on the counterstain.

Uses

  • To identify acid-fast bacteria.
  • To identify pathogenicMycobateriumspp.

Disadvantages

  • Must wear protective goggles when heating carbol fuschin.

Requirements

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Procedure

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Aftercare

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Further Reading

Publications

Refereed papers

  • Recent references from PubMed and VetMedResource.
  • Cray C & Zaias J (2004) Laboratory procedures. Vet Clin Exotic Anim Pract (2), 487-518 PubMed.

Other sources of information

  • Dunn J K & Gerber K (2005) Diagnostic Cytology. In:BSAVA Clinical and Feline Clinical Pathology. 2nd edn. Eds: Villiers E & Blackwood L. BSAVA, Gloucester. pp 305-339.

Organisation(s)

  • Mary Pinborough, Pinmoore Animal Laboratory Services Ltd, The Coach House, Town House Barn, Clotton, Cheshire CW6 0EG, UK. Tel: +44 (0)1829 781855; Email:  sales@palsvetlab.co.uk ; Website:  www.palsvetlab.co.uk .

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