ISSN 2398-2969      

Staining technique: Wright's

Clapis
Contributor(s):

Synonym(s): Romanowsky stain


Introduction

  • A Romanowsky-type stain   Staining technique: Romanowsky stains - overview  .
  • The choice of stain used is dependent on availability of equipment  and staining solutions in practice, the experience and preference of the clinician.
  • Stains can be either applied manually with the aid of cuvettes, or mechanically in automated staining systems.
  • Romanowsky stains are neutral stains compsed of a mixture of oxidized methylene blue (azure) dyes and Eosin Y.
  • The azures are basic dyes that bind acid nuceli and result in a blue to purple color.
  • The eosines are acidic dyes that are attracted to the alkaline cytoplasm, producing red coloration.
  • They generally provide excellent nucelar detail and also clear differentiation of the cytoplasm.
  • Buffer is critical to force the dyes to precipitate out of solution to bind sample material.
  • Wright's stain can be used for non-gynecological clinical material.

Uses

  • Hematology and cytology.
  • Differentiation of blood cells in peripheral blood smears and bone marrow aspirates.
  • Detects intracellular Leishamaniasis on blood smears, lymph node aspirates or in histological preparations in the dog.

Advantages

  • No need for prior fixing of cells if hemofast (a fixative containing neutral methyl alcohol, ie does not contain acetone, therefore there is no need for prior fixing if the smeared slides) is used.

Disadvantages

  • Distilled water must be used for hemofast.
  • Tap water cannot be used as calcium can cause precipitation of eosin into an insoluble calcium salt.

Requirements

This article is available in full to registered subscribers

Sign up now to obtain ten tokens to view any ten Vetlexicon articles, images, sounds or videos, or Login

Procedure

This article is available in full to registered subscribers

Sign up now to obtain ten tokens to view any ten Vetlexicon articles, images, sounds or videos, or Login

Aftercare

This article is available in full to registered subscribers

Sign up now to obtain ten tokens to view any ten Vetlexicon articles, images, sounds or videos, or Login

Outcomes

This article is available in full to registered subscribers

Sign up now to obtain ten tokens to view any ten Vetlexicon articles, images, sounds or videos, or Login

Further Reading

Publications

Refereed papers

Other sources of information

  • Merck KGaA (2013)Cytodiagnosis Staining Methods.Merck Millipore Manual. Website:www.merckmillipore.com.
  • Dunn J K & Gerber K (2005)Diagnostic Cytology. In:BSAVA Clinical and Feline Clinical Pathology. 2nd edn. Eds: Villiers E & Blackwood L. BSAVA, Gloucester. pp 305-339.
  • Sink C A & Feldman B  F (2004)Laboratory Urinalysis and Haematology for the Small Animal Practitioner.New Tenton Media, Wyoming. ISBN: 978-1893441101.

Organisation(s)

  • Mary Pinborough, Pinmoore Animal Laboratory Services Ltd, The Coach House, Town House Barn, Clotton, Cheshire CW6 0EG, UK. Tel: +44 (0)1829 781855; Email:  sales@palsvetlab.co.uk ; Website:  www.palsvetlab.co.uk .

Can’t find what you’re looking for?

We have an ever growing content library on Vetlexicon so if you ever find we haven't covered something that you need please fill in the form below and let us know!

 
 
 
 

To show you are not a Bot please can you enter the number showing adjacent to this field

 Security code