ISSN 2398-2969      

Staining technique: Rapid trichrome

Clapis

Introduction

  • General cytology.
  • Trichrome staining is composed of three or more components, usually two or more dyes with a polyacid differentiator that stains muscle red and collagen green.

Uses

 

Advantages

  • Allows differentiation between cells and surrounding connective tissue.
  • Provides excellent nuclear detail and stains cytoplasm.

Disadvantages

  • Requires careful technique.
  • Not practical for veterinary practice laboratories.
  • Refer to external laboratory.

Preparation

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Procedure

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Further Reading

Publications

Refereed papers

Organisation(s)

  • Mary Pinborough, Pinmoore Animal Laboratory Services Ltd, The Coach House, Town House Barn, Clotton, Cheshire CW6 0EG, UK. Tel: +44 (0)1829 781855; Email:  sales@palsvetlab.co.uk ; Website:  www.palsvetlab.co.uk .

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