ISSN 2398-2969      

Staining technique: Perl's Prussion Blue/Safranin

Clapis
Contributor(s):

Introduction

  • Potassium ferric ferrocyanide, a colored pigment is formed from the combination of hemosiderin and potassium ferrocyanide.
  • Background stain and cells stain dark pink, any material contianing iron stains blue.

Uses

  • Demonstrate the presence of iron in biopsy samples.
  • To classify the cause of anemia:
    • Evaluates iron stores in bone marrow.
    • Anemia due to iron deficiency has decreased bone marrow storage of iron.
    • Hemolysis or decreased production, have increased bone marrow iron stores.
  • Evalutates siderocytes/sideroblasts - red blood cells with iron that appear as focal areas of basophilic stippling.
  • Evaluates leukocytes containing iron positive inclusions (sideroleukocyte).
  • Evaluates hemosiderin in macrophages from tracheal wash/bronchoalveolar lavage or body cavity fluids. Will indicate previous hemorrhage if iron is identified in macrophages.

This is not performed in general practice and it is advised to send samples to a referral laboratory.

  • 1% acidic potassium ferrocyanide solution (0.1% safranin solution) must always be freshly prepared due to a very short shelf-life.
  • 2% potassium ferrocyanide must be kept in a brown bottle, and stored in a dark cupboard to keep it stable.

Strong acids - biohazard.

Requirements

This article is available in full to registered subscribers

Sign up now to obtain ten tokens to view any ten Vetlexicon articles, images, sounds or videos, or Login

Procedure

This article is available in full to registered subscribers

Sign up now to obtain ten tokens to view any ten Vetlexicon articles, images, sounds or videos, or Login

Further Reading

Publications

Refereed papers

Organisation(s)

  • Mary Pinborough, Pinmoore Animal Laboratory Services Ltd, The Coach House, Town House Barn, Clotton, Cheshire CW6 0EG, UK. Tel: +44 (0)1829 781855; Email:  sales@palsvetlab.co.uk ; Website:  www.palsvetlab.co.uk .

Want more related items, why not
contact us

Can’t find what you’re looking for?

We have an ever growing content library on Vetlexicon so if you ever find we haven't covered something that you need please fill in the form below and let us know!

 
 
 
 

To show you are not a Bot please can you enter the number showing adjacent to this field

 Security code