ISSN 2398-2969      

Staining technique: Periodic acid / schiff


Synonym(s): PAS


  • Stain reacts with structures containing a carbohydrate component (1:2 glucol grouping, including polysaccharides, glycoproteins and glycolipids).
  • Periodic acid is the oxidant, others can be used then results may vary slightly.


  • Identification of fungal hypae, fungal spores and yeasts.
  • Demonstrates glycogen in cells, mucus in various cells and tissues, the basement membrane that underlies epithelia and reticular fibers in connective tissue.


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Further Reading


Refereed papers

Other sources of information

  • Dunn J K & Gerber K (2005) Diagnostic Cytology. In:BSAVA Clinical and Feline Clinical Pathology. 2nd edn. Eds: Villiers E & Blackwood L. BSAVA, Gloucester. pp 305-339.
  • Ross M H, Romrell L J & Kaye G I (1995) Histology A Text and Atlas. 3rd edn. Williams & Wilkins, Baltimore.


  • Mary Pinborough, Pinmoore Animal Laboratory Services Ltd, The Coach House, Town House Barn, Clotton, Cheshire CW6 0EG, UK. Tel: +44 (0)1829 781855; Email: ; Website: .

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