ISSN 2398-2969      

Staining technique: Leishman

Clapis

Synonym(s): Romanowsky stain


Introduction

  • A Romanowsky-type stain   Staining technique: Romanowsky stains - overview  , used in microscopy for staining blood smears.
  • Romanowsky stains are neutral stains composed of a mixture of oxidized methylene blue (azure) dyes and Eosin in alcohol, which is usually diluted and buffered before use.
  • It is generally used to differentiate and identify leukocytes and stains blood components in a range of shades between red and blue.

Uses

  • Hematology: stains WBC, RBC and platelets.

Advantages

  • Provides excellent stain quality.
  • Good cytoplasmic: nuclear contrast.

Disadvantages

  • Bright light and heat oxidize the stain; must be stored in an air tight, semi-opaque container.
  • Slides fade with time and therefore cannot be reliably archived.
  • Refer to external laboratory for staining.

Requirements

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Procedure

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Further Reading

Publications

Refereed papers

Other sources of information

  • Merck KGaA (2013) Cytodiagnosis Staining Methods. Merck Millipore Manual. Website:www.merckmillipore.com.

Organisation(s)

  • Mary Pinborough, Pinmoore Animal Laboratory Services Ltd, The Coach House, Town House Barn, Clotton, Cheshire CW6 0EG, UK. Tel: +44 (0)1829 781855; Email:  sales@palsvetlab.co.uk ; Website:  www.palsvetlab.co.uk .

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