Felis ISSN 2398-2950

Blood smear

Contributor(s): Stephen Barr, Karen L Gerber, Clare Knottenbelt

Introduction

Uses

Indicated in:

  • Classify and characterize anemia Anemia: overview , bleeding disorders(thrombocytopenia)   Thrombocytopenia  or neoplasia (leukemia Leukemia , clinical staging of lymphoma).
  • Suspected inflammatory disease (of infectious or immune-mediated origin).
  • Suspect cases of infection/parasitic disease including:
    • Protozoal parasites: Babesia felis  Babesia felis , Cytauxzoon felis  Cytauxzoon felis .
    • Mycoplasma parasites: Mycoplasma haemofelis and Mycoplasma haemominutum (both subspecies of an organism previously known aHaemobartonella felis).
    • Bacteria: Bartonella spp Bartonella .
  • As a critical adjunct to in-house hematology analyzers, since differential counts on these machines are only a rough estimation.

Advantages

  • Very rapid simple technique requiring minimal equipment.
  • Can be performed with very small volumes of blood.
  • Accurate assessment of cell morphology which may be affected by storage or transport.

Disadvantages

  • Must use clean slides to avoid holes appearing in the smear (grease spots).
  • Smear must be rapidly air dried to ensure that cell morphology is preserved.
  • technique must be used regularly to make useful blood smears.

Requirements

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Preparation

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Procedure

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Aftercare

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Outcomes

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Further Reading

Publications

Refereed papers

Other sources of information

  • Harvey J W (2001) Atlas of Veterinary Hematology. W.B. Saunders Company.
  • Reagan J W (1998) Veterinary Hematology Atlas of Common Domestic Species, Iowa State Press.
  • Duncan JR, Prasse KWet al Veterinary Laboratory Medicine Clinical pathology, third edition, Iowa State University Press
  • Davidson M G, Else R W & Lumsden J H (1998) In:BSAVA Manual of Clinical Pathology. BSAVA, Cheltenham.
  • Meyer D & Harvey J (1998) Interpretation and Diagnosis. In: Veterinary Laboratory Medicine. 2nd edn. Philadelphia: W B Saunders.
  • Willard M D, Tvedten H & Turnwald G H (1994) In: Small Animal Clinical Diagnosis by Laboratory Methods. 2nd edn. Philadelphia: W B Saunders.
  • Jain N C (1993) In: Essentials of Veterinary Hematology. Ed N C Jain. Philadelphia: Lea & Febiger.
  • Bush B M (1975) In: Veterinary Laboratory Manual. London: William Heinemann Medical Books Ltd.


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