ISSN 2398-2950      

Serum protein electrophoresis

ffelis
Contributor(s):

Kathleen P Freeman


Overview

  • At pH 8.6 all serum proteins carry negative charge and migrate from cathode to anode.
  • Proteins can be separated in an electric field on the basis of their molecular charge densities and weight.

Sampling

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Tests

Methodologies

  • Cellulose acetate membrane commonly used.
  • Agarase gel used for high-resolution electrophoresis.

Availability

  • Widely available at commercial laboratories.

Result Data

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Further Reading

Publications

Refereed papers

  • Recent references from VetMedResource and PubMed.
  • Pederson N C (1976) Feline infectious peritonitis, something old, something new. Feline Pract 6, 42-51.

Other sources of information

  • Ettinger S J & Feldman E C (2000) Eds. Textbook of Veterinary Internal Medicine. 5th edn. W B Saunders & Co, USA.
  • Kaneko J J, Harvey J W & Brass M L (1997) Eds. Clinical Biochemistry of Domestic Animals. 5th edn. Academic Press, USA.
  • Duncan J R, Prasse K W & Mahaffey E A (1994) Veterinary Laboratory Medicine. Clinical Pathology. 3rd edn. Iowa University Press, USA.

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