Felis ISSN 2398-2950

Feline parvovirus test

Synonym(s): Feline infectious enteritis, Feline panleukopenia, Feline parvovirus FIE, FPLV, FPV

Contributor(s): David Godfrey, Kim Willoughby

Overview

Sampling

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Tests

Methodologies

In clinic

  • Feline/canine/mink RIM test and canine parvovirus ELISA Enzyme linked immunosorbent assay (ELISA): sample is mixed with reagents and applied to supplied assay device. After the specified time to result is read by a color change in the test spot or band. Both tests have an integral positive control.
    RIM is preferred as it is more sensitive in feline cases (Addie et al, 1998).

External laboratory

  • Virus isolation: cell culture. Inhibition of normal growth of feline cell line. FPV can be differentiated from canine parvovirus by antigenic analysis using hemagglutination inhibition (HAI), autoagglutination characteristics with porcine erythrocytes or restriction endonuclease (RE) digestion. It is also possible to differentiate vaccinal and wild-type FPV strains at specialist laboratories (eg Neil Greenwood, Intervet UK)
  • Virus antigen detection: immunofluorescence on histological sections.
  • Virus genome detection: PCR on feces or intestinal contents.
  • Histopathology: findings consistent with FIE.
  • Serology: HAI test.

Availability

PCR, virus isolation, serology

  • Feline Virus Unit, University of Glasgow Veterinary School.

Canine parvo ELISA

  • Idexx Laboratories Ltd, Grange House, Sandbeck Way, Wetherby, West Yorkshire LS22 5JU. Tel 01507 523276 website:  www.idexx.com 

Feline/canine/mink parvovirus RIM

  • MegaCor Diagnostik GmbH, Lochauer Str. 2 A - 6912 Horbranz, AUSTRIA Tel.:+43 5573 85400, Fax.:+43 5573 85402, e-mail: MegaCor@t-online.de, website:  www.megacor.at 

Validity

Sensitivity

  • All tests except the canine parvo are highly sensitive (>95%). This does, however, depend on when during the course of clinical disease the sample is collected. Clinical signs will often persist after virus is detectable by antigen testing in the feces.

Specificity

  • False positive may occur with recent vaccination or carriage of environmental virus through the GIT of immune cats, eg in an endemically infected cattery.
  • Fecal antigen tests are not specific for FPV but detect canine, feline or mink Parvovirus.

Predictive value

  • Good, in combination with clinical findings.

Result Data

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Further Reading

Publications

Refereed papers

  • Recent references from VetMed Resource and PubMed.
  •  Esfandiari J & Klingeborn B (2000) A comparative study of a new rapid and one-step test for the detection of parvovirus in feces from dogs, cats and mink. J Vet Med B Infect Dis Vet Public Health 47, 145-153.
  • Addie D D, Toth S, Thompson H, Greenwood N & Jarrett 0 (1999) Detection of feline parvovirus in dying pedigree kittens. Vet Rec 142, 353-356.


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