ISSN 2398-2950      

Feline pancreatic lipase immunoreactivity (fPLI) test

ffelis

Overview

  • fPLI  is an acronym for feline Pancreatic Lipase Immunoreactivity, a specific lipase found only in feline pancreatic tissue.
  • The commercially available assay measures concentrations of pancreatic lipase in the serum.
  • Inflammation of the pancreas leads to an increased release of pancreatic lipase into the circulation, thus elevated concentrations of pancreatic lipase are consistent with a diagnosis of pancreatitis Pancreatitis.

Sampling

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Tests

Methodologies

  • Serum feline pancreatic lipase immunoreactivity is measured with an ELISA Enzyme linked immunosorbent assay (ELISA).
  • This test is available exclusively from the Gastrointestinal Laboratory at the Texas A&M University.
    • A microplate is coated with monoclonal antibodies against feline pancreatic lipase.
    • Sera are diluted and incubated on the plate, allowing the monoclonal antibodies to capture the pancreatic lipase, while other proteins are washed away.
    • A secondary antibody carrying a label is then incubated in the wells.
    • The amount of bound label is proportionate to the quantity of pancreatic lipase in the original serum sample.
    • A standard curve is constructed using a recombinant peptide that is part of the native canine pancreatic lipase protein.

Validity

Sensitivity

  • Determination of feline pancreatic lipase in serum has a sensitivity of 82% for the diagnosis of pancreatitis.

Specificity

  • Determination of feline pancreatic lipase generally has >95% specificity for the diagnosis of pancreatitis. Specificities of 100% have been reported in some studies.

Technique (intrinsic) limitations

  • Avoid submission of severely lipemic or hemolyzed samples.

Result Data

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Further Reading

Publications

Refereed papers

  • Recent references from VetMedResource and PubMed.
  • Forman M A, Marks S L, De Cock H E V, Hergesell E J, Wisner E R, Baker T W, Kass P H, Steiner J M & Williams D A (2004) Evaluation of serum feline pancreatic lipase immunoreactivity and helical computed tomography versus conventional testing for the diagnosis of feline pancreatitis. J Vet Intern Med 18 (6), 807-815 PubMed.
  • Steiner J M, Wilson B G & Williams D A (2004) Development and analytical validation of a radioimmunoassay for the measurement of feline pancreatic lipase immunoreactivity in serum. Can J Vet Res 68 (4), 309-314 PubMed.
  • Steiner J M, Wilson B G & Williams D A (2003) Purification and partial characterization of feline classical pancreatic lipase. Comp Biochem Physiol B Biochem Mol Biol 134 (1), 151-159.

Organization(s)

  • The Gastrointestinal Laboratory, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station TX 77845-4474, USA;  www.cvm.tamu.edu/gilab.

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