ISSN 2398-2950      

Cerebrospinal fluid: cytology

ffelis

Synonym(s): CSF: cytology


Overview

Sampling

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Tests

Methodologies

  • Total cell count with hemocytometer.
  • Or use cytocentrifuge for concentration of cells.
  • Air dry slide.
  • Direct smears only useful for extremely cellular samples.

Availability

  • Sedimentation technique: standard.
  • Cytocentrifuge: specialist laboratory.

Technique (intrinsic) limitations

  • CSF samples can be contaminated with blood.
  • CSF cytology may change as CNS illness progresses.

Technician (extrinsic) limitations

  • Blood contamination more consistent feature of inexperienced operator.
  • Some experience is necessary in interpreting cell types in cytologic preparations.

Result Data

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Further Reading

Publications

Refereed papers

  • Recent references from VetMedResource and PubMed.
  • Singh M, Foster D J, Child G & Lamb W A (2005) Inflammatory cerebrospinal fluid analysis in cats: clinical diagnosis and outcome. J Feline Med Surg 7, 77-93 PubMed.
  • Mayhew I G & Beal C R (1989) Techniques in analysis of cerebrospinal fluid. In - Symposium on Advances in Veterinary Neurology. VCNA 10, 155-176.

Other sources of information

  • Cowell R L, Tyler R D & Meinkoth J H (1999) Diagnostic Cytology and Hematology of the Dog and Cat. 2nd edn. Mosby, USA.
  • Duncan J R, Prasse K W & Mahaffey E A (1994) Veterinary Laboratory Medicine. Clinical Pathology. 3rd edn. Iowa University Press, USA.
  • Brobst D & Bryan G (1989) Cerebrospinal fluid. In. Diagnostic Cytology of the Dog and Cat.1st edn. Eds: R L Cowell and R D Tyler. American Veterinary Publications Inc. pp 141-148.

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