ISSN 2398-2950      

Blood smear: staining for rbc parasites

ffelis
Contributor(s):

Severine Tasker


Overview

  • Staining of blood smears to reveal erythrocyte associated parasites.

Sampling

This article is available in full to registered subscribers

Sign up now to obtain ten tokens to view any ten Vetlexicon articles, images, sounds or videos, or Login

Tests

Availability

  • Blood smear preparation and staining can be performed in-house.

Validity

Sensitivity

  • Blood smear examination for H. felis detection is less sensitive than a polymerase chain reaction (PCR) assay PCR (Polymerase chain reaction).
  • Only 37.5% of blood smears were H. felis cytology positive in experimentally H. felis inoculated PCR positive cats.
  •  H. felis infected cats exhibit cyclical parasitemia so false negative cytological diagnoses can occur.
  • Examination of a series of smears made over several days may increase sensitivity.

Specificity

  • False positive diagnoses can occur due to stain precipitate, Howell-Jolly bodies, Pappenheimer bodies, refractile artifacts and erythrocyte crenation.

Technique (intrinsic) limitations

  • Blood smear examination is not adequate for clear differentiation between strains of H. felis - PCR is required to determine strain type.

Result Data

This article is available in full to registered subscribers

Sign up now to obtain ten tokens to view any ten Vetlexicon articles, images, sounds or videos, or Login

Further Reading

Publications

Refereed papers

  • Recent references from VetMedResource and PubMed.
  • Bobade P A & Nash A S (1987) A comparative study of the efficiency of acridine orange and some Romanowsky staining procedures in the demonstration of Haemobartonella felis in feline blood. Vet Parasitol 26, 169-172.
  • Foley J E, Harrus S, Poland A, Chomel B & Pedersen N C (1998) Molecular, clinical, and pathologic comparison of two distinct strains of Haemobartonella felis in domestic cats. Am J Vet Res 59, 1581-1588.
  • VanSteenhouse J L, Millard J R & Taboada J (1993) Feline haemobartonellosis. Comp Cont Ed Pract Vet 15, 535-545.
  • Westfall D S, Jensen W A, Reagan W J, Radecki S V & Lappin M R (2001) Inoculation of two genotypes of Haemobartonella felis (California and Ohio variants) to induce infection in cats and the response to treatment with azithromycin. Am J Vet Res 62, 687-691.

Related Images

MEMBER BENEFIT

Can’t find what you’re looking for?

We have an ever growing content library on Vetlexicon so if you ever find we haven't covered something that you need please fill in the form below and let us know!

 
 
 
 

To show you are not a Bot please can you enter the number showing adjacent to this field

 Security code