Felis ISSN 2398-2950

Anti-platelet antibody

Contributor(s): Elizabeth Villiers

Overview

  • Anti-megakaryocyte antibody.
  • Anti-platelet antibody test.

Sampling

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Tests

Methodologies

Platelet-associated antibody tests

  • Direct immunofluorescence, flow cytometry.
  • Direct methods: use whole blood, EDTA sample.
  • Indirect methods: sample serum or plasma tested against pooled normal platelets.

Anti-megakaryocyte antibody test

  • Detects megakaryocyte-associated immunoglobulin.
  • Positive test may also suggest presence of antibodies against platelets.

Availability

  • Limited in UK.
  • Cambridge University Vet School, in the UK, performs direct antibody test (EDTA sample).
    Turnaround often slow, hence limited diagnostic use but valuable as confirmatory test.

Validity

Sensitivity

  • Direct anti-platelet antibody tests: low sensitivity due to low numbers of platelets.
  • Indirect tests: less sensitive.

Specificity

  • Indirect tests: false positives in patients with other immune-mediated diseases, non-immune-mediated thrombocytopenia, neoplasia.

Technique (intrinsic) limitations

  • Confirmation of immune-mediated disease.

Result Data

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Further Reading

Publications

Refereed papers

  • Recent references from VetMedResource and PubMed.
  • Day M J (1999) Clinical immunology of the dog and cat. London: Manson Publishing. ISBN: 1 8745 4598 7.
  • Day M J (1996) Diagnostic assessment of the feline immune system, part 2. Feline Practice 24(3), 14-25.


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