ISSN 2398-2950      

Anti-nuclear antibody

ffelis
Contributor(s):

Michael Day

Synonym(s): ANA test


Overview

Sampling

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Tests

Methodologies

Indirect immunofluorescence or immunoperoxidase

  • Dilutions of patient's serum (from 1:5 to 1:1000) incubated with mouse/rat liver or tissue culture cells.
  • If present, anti-nuclear antibodies bind to substrate nuclei.
  • ANA detected by fluorescein-conjugated anti-feline globulin (peroxidase-conjugated system may also be used).
  • Titer: highest dilution of patient's serum causing staining of nuclei.
  • Positive titer depends on cell substrate and laboratory; usually 1:40 to 1:100.

Validity

Sensitivity

  • High.

Specificity

  • Poor since many other diseases (eg chronic inflammation or neoplasia) may have ANA titer; but this is usually lower than in SLE or immune-mediated disease.

Predictive value

  • Not reported.

Technique (intrinsic) limitations

  • Absolute diagnosis of SLE requires a positive ANA test and at least two major clinical signs of SLE.

Technician (extrinsic) limitations

  • Reading of immunofluorescence results requires experience.
  • Normal values vary between laboratories.

Result Data

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Further Reading

Publications

Refereed papers

  • Recent references from PubMed and  VetMedResource.
  • Day M J (1996) Diagnostic assessment of the feline immune system, part 2. Feline Pract 24, 14-25.

Other sources of information

  • Day M J (1999) Clinical Immunology of the Dog and Cat. Manson Publishing, UK. ISBN: 1 8745 4598 7.

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