ISSN 2398-2977      

Blood smear

pequis
Contributor(s):

Clare Knottenbelt


Introduction

  • Blood smears are simple to perform and can provide useful information on all blood cell lines.
  • Platelet counts, white blood cell differential counts and morphology, red blood cell morphology.
  • Characterization of hematologic disorders which will assist in definitive diagnosis.

Uses

  • Indicated in:
    • Suspected cases of anemia, thrombocytopenia or leukemia.
    • Suspected inflammatory disease (of infectious or immune-mediated origin).
    • As an adjunct to in-house hematology analyzers.

Advantages

  • Very rapid simple technique requiring minimal equipment.
  • Can be performed with very small volumes of blood.
  • Accurate assessment of cell morphology which may be affected by storage or transport.

Disadvantages

  • Must use clean slides to avoid holes appearing in the smear (grease spots).
  • Smear must be rapidly air dried to ensure that cell morphology is preserved.

Requirements

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Preparation

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Procedure

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Aftercare

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Outcomes

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Further Reading

Publications

Refereed papers

Other sources of information

  • Davidson M G, Else R W & Lumsden J H (1998) In: BSAVA Manual of clinical pathology. BSAVA, UK.
  • Meyer D and Harvey J (1998) Interpretation and Diagnosis. In: Veterinary Laboratory Medicine. 2nd edition. W B Saunders, USA.
  • Willard M D, Tvedten H & Turnwald G H (1994) Small Animal Clinical Diagnosis by Laboratory Methods. 2nd edition. W B Saunders, USA.
  • Jain N C (1993) Essentials of Veterinary Hematology. Eds: Jain N C. Lea & Febiger, USA.
  • Bush B M (1975) Veterinary Laboratory Manual. William Heinemann Medical Books Ltd, UK.

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