ISSN 2398-2977      

Respiratory: lower airway cytology

pequis

Overview

  • Identification of cell types in respiratory secretions is used in the diagnosis and characterization of respiratory disease.
  • Cytology of fluids from the lower respiratory tract provides information about the condition of tissues next to the air spaces.
Print off the Owner factsheet on Diagnostic tests to give to your clients.

Sampling

This article is available in full to registered subscribers

Sign up now to obtain ten tokens to view any ten Vetlexicon articles, images, sounds or videos, or Login

Tests

Methodologies

  • Centrifugation/cytocentrifugation onto slide.
  • Air-dry smears as quickly as possible, using a fan if necessary.
  • Stain with Diff-Quik, Wright-Giemsa, May-Gruenwald for routine cytology.
  • Use Prussian blue for hemosiderin, or Toluidine blue for mast cells.
  • Examine at high power (900-1000x) and >500 cells.

Technician (extrinsic) limitations

  • Fluid collected by transendoscopic or transtracheal aspirate will reflect a different cell population than that collected by bronchoalveolar lavage.
  • The volume of fluid instilled and collected will influence the proportion of cells observed; smaller volumes result in higher mast cell and neutrophil counts.

Result Data

This article is available in full to registered subscribers

Sign up now to obtain ten tokens to view any ten Vetlexicon articles, images, sounds or videos, or Login

Further Reading

Publications

Refereed papers

  • Recent references fromPubMedpublished during the last 12 months.
  • Raidal S L, Love D N, Bailey G D & Rose R J (2000)The effect of high intensity exercise on the functional capacity of equine pulmonar alveolar macrophages and BAL-derived lymphocytes. Res Vet Sci68(3), 249-253PubMed.
  • Christley R M, Hodgson D R, Rose R J, Reid S W J & Hodgson J L (1999)Comparsion of bacteriology and cytology of tracheal fluid samples collected by percutaneous transtracheal aspiration or via an endoscope using a plugged, guarded catheter. Equine Vet Educ31(3), 197-202PubMed.
  • Hoffman A M (1999)Bronchoalveolar lavage technique and cytological diagnosis of small airway inflammatory disease. Equine Vet Educ11, 330-336.

Related Images

RELATED FACTSHEETS

Diagnostic tests

MEMBER BENEFIT

Can’t find what you’re looking for?

We have an ever growing content library on Vetlexicon so if you ever find we haven't covered something that you need please fill in the form below and let us know!

 
 
 
 

To show you are not a Bot please can you enter the number showing adjacent to this field

 Security code