Canis ISSN: 2398-2942

Thyroid stimulating hormone assay

Synonym(s): TSH assay

Contributor(s): David Bruyette

Overview

  • Thyroid stimulating hormone is produced in the anterior pituitary and regulates the synthesis and secretion of thyroxine (T4) and tri-iodothyronine (T3) Hypothalamic-pituitary-thyroid axis diagram.

Sampling

This article is available in full to registered subscribers

Sign up now to purchase a 30 day trial, or Login

Tests

Methodologies

  • Enzyme-linked immunosorbent assay or immunoradiometric.

Availability

  • Most commercial laboratories.

Validity

Sensitivity

  • 75%.

Specificity

  • 95-100% for normal dogs.
  • 70% for sick-euthyroid dogs.

Predictive value

  • Positive predictive value 55-75% (depending on which group of dogs you compare to).
  • Negative predictive value 90-100% (depending on which group of dogs you compare to).

Technique (intrinsic) limitations

  • Cannot reliably distinguish low and normal concentrations of cTSH.

Technician (extrinsic) limitations

  • Minimal risk of technician errors.

Result Data

This article is available in full to registered subscribers

Sign up now to purchase a 30 day trial, or Login

Further Reading

Publications

Refereed papers

  • Recent references from VetMedResource and PubMed.
  • Dixon R M &Mooney C T (1999) Evaluation of serum free thyroxine and thyrotropin concentrations in the diagnosis of canine hypothyroidism. JSAP 40(2), 72-78.
  • Panclera D L (1999)Is it possible to diagnose hypothyroidism? JSAP 40(4), 152-157.
  • Iversen L, Hoier R, Jensen A L et al(1998) Evaluation of the analytical performance of an enzyme immunometric assay (EIA) designed to measure endogenous thyroid-stimulating hormone (TSH) in canine serum samples. JAVMA 45, 93-98.
  • Bruner J M, Scott-Moncrieff C R & Williams D A (1998) Effect of time of sample collection on serum thyroid-stimulating hormone concentrations in euthyroid and hypothyroid dogs. JAVMA 212, 1572-1575.
  • Peterson M E, Melian C & Nichols R (1997) Measurement of serum total thyroxine, triodothyronine, free thyroxine and thyrotropin concentrations for diagnosis of hypothyroidism in dogs. JAVMA 211, 1396-1402.
  • Ramsey I K, Evans H & Herrtage M E (1997) Thyroid stimulating hormone and total thyroxine concentrations in euthyroid, sick euthyroid and hypothyroid dogs. JSAP 38(12), 540-545.
  • Dixon R M, Graham P A & Mooney C T (1996) Serum thyrotropin concentrations - a new diagnostic test for canine hypothyroidism. Vet Rec 138, 594-595.

Other sources of information

  • Information available from commercial laboratories who perform the test.


ADDED