ISSN 2398-2942      

Progesterone assay

icanis

Overview

  • Progesterone responsible for secretory changes in the endometrium.
  • Secreted by the corpus luteum, testis (Leydig cells) and adrenal cortex but not the placenta; the dog is the only know domestic animal species that does not exhibit placental steroidogenesis.  
  • Progesterone concentrations are low in proestrus.
  • Rises sharply after the luteinizing hormone (LH) surge and are elevated at ovulation.
  • Remains elevated during the pregnant and non-pregnant diestrus.

Sampling

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Tests

Methodologies

  • RIA → accurate and time consuming, slow turnaround time.
  • Fluorescence enzyme immunoassays → rapid, user friendly interface; several equipment available for in-house use.
  • In-house ELISA kits → semi-quantitative, fast, inexpensive.

Availability

  • Widely available.

Result Data

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Further Reading

Publications

Refereed papers

  • Recent references from VetMed Resource and PubMed.
  • Hollinshead F K, Hanlon  DW (2019) Normal progesterone profiles during estrus in the bitch: A prospective analysis of 1420 estrous cycles. Theriogenology 125, 37-42 PubMed
  • Nöthling J O, De Cramer K G (2019) Comparison of progesterone assay by chemiluminescence or radioimmunoassay for clinical decision-making in canine reproduction. J S Afr Vet Assoc 90(1), 1-6 PubMed
  • Arlt S (2018) Canine ovulation timing: A survey on methodology and an assessment on reliability of vaginal cytology. Reprod Domest Anim 53, 53-62 PubMed

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