Canis ISSN: 2398-2942

Fecal analysis: microscopy

Contributor(s): Helen Milner, James Simpson

Overview

  • Examination of fresh fecal samples for the presence of undigested starch, fat or muscle fiber Fecal analysis: overview.
  • No such material should be present in health.

Sampling

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Tests

Methodologies

  • Collect fresh feces in a suitable container.
  • Using a spatula apply a thin film of feces to 2 microscope slides.
  • Place slides on staining rack.
  • Flood 1 slide with Lugol's iodine Fecal analysis: Lugol's iodine and the other with Sudan 3 stain Fecal analysis: Sudan 3.
  • Leave for 5 minutes.
  • Decant excess stain.
  • Dry underside of slide.
  • View under low power (eg x4) microscopy for black starch granules (Lugols stain) and for red fat granules (Sudan 3 stain).

Disposal

  • Dispose of consumables and fecal material following Health and Safety regulations.

Technician (extrinsic) limitations

  • Test procedures easily carried out in practice laboratory with little additional technical skill required.

Result Data

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Further Reading

Publications

Refereed papers

Other sources of information

  • Manual of Small Animal Clinical Pathology (1998) Davidson M, Else R & Lumsden L (eds), BSAVA, Cheltenham.
  • Clinical Textbook for Veterinary Technicians (1998) 4th edn. McCurnin (ed), W B Saunders.
  • Laboratory Procedures for Veterinary Technicians (1997) 3rd edn Pratt (ed), Mosby.


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