Canis ISSN: 2398-2942

Cerebrospinal fluid: protein

Contributor(s): Vetstream Ltd

Overview

  • Total protein and protein fractions vary with type of central nervous sytem (CNS) pathology and etiology.
  • Normally low, increased protein in the cerebrospinal fluid (CSF) may be due to altered permeability of the blood-brain barrier (mainly albumin) and/or local immunoglobulin production.

Sampling

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Tests

Methodologies

  • Precipitation and dye-binding methods.
  • Urinary protein reagent strips for detection of grossly elevated total protein.
  • Protein electrophoresis Serum protein electrophoresis → relative proportions, eg albumin versus globulin, helps identify source of protein: via increased permeability of blood-brain barrier or from local source (production by meninges).
  • Refractometry:
    • A drop of fluid is placed on refractometer plate.
    • Most refractometers are calibrated to give a reading for total protein in g/dl.
    • Refractometry measures the refractive index (RI) of the fluid.
    • The RI changes proportionally to changes in protein concentration.
  • Precipation and dye binding methods.
  • Urinary protein reagent strips for detection of grossly elevated total protein.

Availability

  • Widely available at commecial laboratories.
  • Refractrometry can be done in practice.

Result Data

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Further Reading

Publications

Refereed papers


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