ISSN 2398-2942      

Blood: crossmatching

icanis

Overview

  • Major crossmatch detects antibodies in recipient plasma against donor red cells.
  • Minor crossmatch detects antibodies in donor plasma against recipient red cells (due to dilution effect when plasma given).
  • If no other donors available, blood with least cross match/incompatibility can be used as cells unlikely to be lysed rapidly.
  • Naturally occuring alloantibodies to canine red cells antigens are unusual. First time transfusions seldom indicate cross match incompatibilities in the dog.
  • Compatible transfusion should prevent hemolytic transfusion reactions Anemia: transfusion indications Blood: transfusion Blood types.

Sampling

This article is available in full to registered subscribers

Sign up now to obtain ten tokens to view any ten Vetlexicon articles, images, sounds or videos, or Login

Tests

Methodologies

Major crossmatch

  • 1 drop of donor blood (in EDTA or acid citrate dextrose (ACD)), transferred to small glass tube.
  • Wash red cells at 37°C by adding 1 ml warmed normal saline, centrifuging and pouring off saline.
  • Repeat twice more.
  • Resuspend 0.2 ml of washed red cells in 4.8 ml 0.9% saline.
  • Add 2 drops of recipient serum (or EDTA plasma), to 2 drops of resuspended donor cells.
  • Prepare 3 tubes.
  • Incubate one at 37°C, one at 4°C and one at room temperature for 15 mins, then centrifuge 1000 rpm for 30 secs.
  • Add 1 drop of 20% bovine serum albumin (BSA), and reincubate 15 mins.
  • Check for visible hemolysis or agglutination.
  • Tap tube to resuspend cells and transfer 2 drops to a glass slide and check for evidence of agglutination or rouleaux formation.

Minor crossmatch

  • Donor plasma mixed with washed red cells from recipient and incubated as above.

Availability

  • Widely available at commercial laboratories.
  • Can easily be performed in practice.

Result Data

This article is available in full to registered subscribers

Sign up now to obtain ten tokens to view any ten Vetlexicon articles, images, sounds or videos, or Login

Further Reading

Publications

Refereed papers

  • Recent references from VetMedResource and PubMed.
  • Giger U, Gelen C J, Callan M B & Oakley D A (1995) An acute hemolytic transfusion reaction caused by dog erythrocyte antigen 1.1 incompatibility in a previously sensitized dog. JAVMA 206 (9), 1358-1362.
  • Hale A S (1995) Canine blood groups and their importance in veterinary transfusion medicine. Vet Clin N Am 25 (6), 1323-1332.

MEMBER BENEFIT

Can’t find what you’re looking for?

We have an ever growing content library on Vetlexicon so if you ever find we haven't covered something that you need please fill in the form below and let us know!

 
 
 
 

To show you are not a Bot please can you enter the number showing adjacent to this field

 Security code