Canis ISSN: 2398-2942

Blood biochemistry: vitamin B12

Synonym(s): B12, cobalamin

Contributor(s): Ken Harkin, Craig Ruaux, James Simpson

Overview

  • Absorbed in ileum, therefore malabsorption results in low levels in blood.
  • Utilized by small intestinal bacteria, therefore bacterial overgrowth reduces levels.

Sampling

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Tests

Methodologies

  • Radioimmunoassay.
  • Chemiluminescent immunoassay.
  • Bioassays.

Availability

  • Specialist laboratory.
  • Canine and feline serum may contain binding proteins that need to be denatured and absorbed before measurement via a "charcoal boil" step. Human laboratories may not necessarily carry out this step, by preference use a laboratory that routinely measures vitamin B12 in veterinary species.

Result Data

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Further Reading

Publications

Refereed papers

  • Recent references from VetMed Resource and PubMed.
  • Fyfe J C, Giger U, Hall C A, Jezyk P F, Klumpp S A, Levine J S & Patterson D F (1991) Inherited selective intestinal cobalamin malabsorption and cobalamin deficiency in dogs. Pediatr Res 29(1), 24-31.
  • Simpson K W et al (1989) Effect of exocrine pancreatic insufficiency on cobalamin absorption in dogs. Am J Vet Res 50(8), 1233-1236.

Other sources of information

  • Simpson J W (1998) Gastrointestinal system and exocrine pancreas. In: Manual of Small Animal Clinical pathology. Davidson M, Else R & Lumsden J (eds). BSAVA, Cheltenham.
  • Bush B M (1991) Interpretation of Laboratory Results. Blackwell Scientific Publication, Oxford.


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