Canis ISSN: 2398-2942

Blood biochemistry: trypsin-like immunoreactivity

Synonym(s): Immunoreactive trypsin, IRT, TLI

Contributor(s): Kathleen P Freeman

Overview

  • Trypsin is a pancreatic proteolytic enzyme; if it leaks from pancreatic cells it causes severe necrotizing pancreatitis.
  • Immunoreactive trypsin (probably a trypsin precursor) is present in serum/plasma and will react with anti-trypsin antibodies but has no proteolytic activity.
  • Decreased TLI indicates exocrine pancreatic insufficiency.
  • Increased TLI may be seen with acute pancreatitis.

Sampling

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Tests

Availability

  • Specialist laboratory.
  • Many laboratories may have arrangements with a specialist laboratory to perform this assay.

Validity

Sensitivity

  • Highly sensitive and specific for diagnosis of exocrine pancreatic insufficiency (EPI).
  • Marked decrease in TLI (<2 microg/l) may precede weight loss, diarrhea, or abnormalities in fecal proteolytic activity.

Result Data

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Further Reading

Publications

Refereed papers

  • Recent references from VetMed Resource and PubMed.
  • Williams D A & Batt R M (1988) Sensitivity and specificity of radioimmunoassay of serum trypsin-like immunoreactivity for the diagnosis of canine exocrine pancreatic insufficiency. JAVMA 192, 195.

Other sources of information

  • Ettinger S J & Feldman E C (eds) (2000)T extbook of Veterinary Internal Medicine. 5th edn. Philadelphia: W B Saunders & Co.
  • Kaneko J J, Harvey J W & Brass M L (eds) (1997) Clinical Biochemistry of Domestic Animals. 5th edn. Boston: Academic Press.
  • Duncan J R, Prasse K W & Mahaffey E A (1994) Veterinary Laboratory Medicine. Clinical Pathology. 3rd edn. Iowa: Iowa University Press.


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