Canis ISSN: 2398-2942

Serum protein electrophoresis

Contributor(s): Kathleen P Freeman, Jane Miller

Overview

  • At pH 8.6 all serum proteins carry negative charge and migrate from cathode to anode.
  • Proteins can be separated in an electric field on the basis of their molecular charge densities and weight.

Sampling

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Tests

Methodologies

  • Cellulose acetate membrane commonly used.
  • Agarose gel used for high-resolution electrophoresis.

Availability

  • Widely available at commercial laboratories.

Result Data

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Further Reading

Publications

Refereed papers

  • Font A (1996)Consider leishmaniasis in differential for monoclonal gammopathies in dogs. JAVMA208, 104-107.

Other sources of information

  • Ettinger S J & Feldman E C (eds) (2000)Textbook of Veterinary Internal Medicine.5th edn. Philadelphia: W B Saunders & Co.
  • Kaneko J J, Harvey J W & Brass M L (eds) (1997)Clinical Biochemistry of Domestic Animals.5th edn. Boston: Academic Press.
  • Duncan J R, Prasse K W & Mahaffey E A (1994)Veterinary Laboratory Medicine. Clinical Pathology.3rd edn. Iowa: Iowa University Press.


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