Canis ISSN: 2398-2942

Fecal analysis: Sudan 3

Contributor(s): Helen Milner, James Simpson

Overview

  • Sudan 3 can be used to stain fresh fecal samples for the detection of undigested fat (steatorrhea) Fecal smear: fat globules Fecal analysis: overview.
    Undigested fat should not normally be found in fecal smears.

Sampling

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Tests

Methodologies

  • Collect fresh fecal sample in a suitable container.
  • Using a spatula apply thin film of feces to a microscope slide.
  • Place on a staining rack.
  • Flood stain with Sudan 3 stain.
  • Leave for 5 minutes.
  • Decant excess stain.
  • Dry underside of slide.
  • View under low power (eg x4) microscopy for red/orange fat granules.

Dispose of consumables and fecal material following Health and Safety regulations.

Result Data

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Further Reading

Publications

Refereed papers

Other sources of information

  • Manual of Small Animal Clinical Pathology (1998) Davidson M, Else R & Lumsden J (eds), BSAVA.
  • Clinical Textbook for Veterinary Technicians (1998) 4th edn McCurnin D M (ed), W B Saunders.
  • BSAVA Manual of Canine and Feline Gastroenterology (1997) Thomas D A et al(eds), Iowa State University Press.
  • Laboratory Procedures for Veterinary Technicians (1997) 3rd edn, Pratt P W (ed), Mosby.
  • Williams D A (1985) The diagnosis of canine pancreatic exocrine insufficiency. In: The Veterinary Annual. Grunsell C S G, Hill F W G & Raw M E (eds), Scientechnica, Bristol. pp 330.


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